Molecular configuration of amylose and its complexes in aqueous solutions. Part III. Investigation of the DP distribution of helical segments in amylose–iodine complexes
作者:
J. Szejtli,
S. Augustat,
M. Richter,
期刊:
Biopolymers
(WILEY Available online 1967)
卷期:
Volume 5,
issue 1
页码: 17-26
ISSN:0006-3525
年代: 1967
DOI:10.1002/bip.1967.360050104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractThe equilibrium normality [If] of free iodine in amylose‐iodine complex formation is a function of the length of the polyiodine chains. This length depends on theDPof helical segments of amylose (sDPn). Values of [If] and of the concentration of the bound iodine [Ib] were determined by the continuous photometric titration with automatic recording. Plots of [Ib] versus [If] give an integral distribution curve. Since the relation between [If] andsDPnis known, the graphic differentiation of the [Ib] versus [lf] curve furnishes the differential distribution curve, representing the mass distribution of the helical segments according to theirDP. The peak of this curve is characteristic of the percentage and DP of those helical segments, which occur in the largest amount. On the basis of the differential distribution curve the polymolecularity of the investigated sample may be judged. The titration of amylose samples degraded by various methods gives different distribution curves. Titrating mixtures of samples with widely differing average DP values results in differential curves having more than one maximu
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