The positron emitters18F and11C have been evaluated for their suitability in whole-body autoradiography (WBA). The autoradiographic representation of the biodistribution of 218F-fluoro-2-deoxy-D-glucose (18FFDG).18FNaF and L-S-11C-methyl-methionine is given as an example. It was possible to obtain good autoradiographic pictures of these compounds in spite of the short physical half-time by modifying the whole-body autoradiographic technique. The modified WBA provides a valuable method in biodistribution studies on animals of radiopharmaceuticals for use in positron emission tomography (PET).