Detection of Rectal Antibodies to HIV‐1 by a Sensitive Chemiluminescent Western Blot Immunodetection Method
作者:
Omari Mohamed,
Rhoda Ashley,
Andrew Goldstein,
Julie McElrath,
Julie Dalessio,
Lawrence Corey,
期刊:
Journal of Acquired Immune Deficiency Syndromes
(OVID Available online 1994)
卷期:
Volume 7,
issue 4
页码: 375-380
ISSN:0894-9255
年代: 1994
出版商: OVID
关键词: HIV-1;Rectal antibodies;Local immunity.
数据来源: OVID
摘要:
SummaryWestern blot with a time-dependent enhanced chemiluminescence immunodetection method (ECL-WB) was shown to be 100-fold more sensitive than standard commercial colorimetric Western blots (WB) for detecting serum IgG to human immunodeficiency virus type I (HIV-1). ECL-WB was then used to test rectal secretions from 15 HIV-1 infected subjects (HIV +) and 7 uninfected subjects (HIV-) to document local IgG, IgA, and secretory component-associated immunoglobulin (SC-Ig) to HIV-1 proteins. Fourteen of 15 HIV+ subjects had rectal IgA to at least 1 HIV-1 protein, most often to gp41 (80%) or p24 (60%) and 14 (93%) had IgG to gp160, gp120, or gp41. Of seven HIV - subjects, none had detectable bands to HIV-1 proteins. SC-Ig to HIV-1 proteins was detected in all five rectal samples tested. However, the antibody profiles differed from those of rectal IgA, suggesting more than one source of rectal IgA to HIV. ECL-WB requires individual optimization and interpretation for each specimen as well as expensive reagents and is, therefore, not currently applicable to screening assays. However, the method offers promise as a sensitive method to characterize low-level immune responses (IgG, IgA, and SC-Ig) to HIV-1 proteins at local sites such as rectal mucosae.
点击下载:
PDF
(521KB)
返 回