The location of principal and accessory motoneurons and principal interneurons of the nucleus abducens was determined in the Caspian terrapin (Mauremys caspica) by means of horseradish peroxidase histochemical tracing. Enzyme injections were made into the ipsilateral lateral rectus and retractor bulbi muscles and into the contralateral oculomotor nucleus. Labeled principal abducens motoneurons formed a cluster of cells in the rhombencephalon, under the IVth ventricle and adjacent to the medial longitudinal fascicle. The accessory abducens motoneurons were located more deeply in the rhombencephalon and more ventrolaterally than the principal motoneurons forming a compact aggregation of neurons. The principal interneurons of abducens nucleus were arranged as a cluster of cells under the floor of the IVth ventricle and more lateral than the principal motoneurons, with no intermingling.