首页   按字顺浏览 期刊浏览 卷期浏览 Optimization of Total Triiodothyronine Radioimmunoassay in Un-extracted Serum by Dextra...
Optimization of Total Triiodothyronine Radioimmunoassay in Un-extracted Serum by Dextran Charcoal

 

作者: Y. M. Megahed,   G. A. Ziada,   M. M. Mahfouz,   M. Abdel-Razak,   S. El-Hadad,  

 

期刊: Isotopenpraxis Isotopes in Environmental and Health Studies  （Taylor Available online 1977）
卷期: Volume 13, issue 8  

页码: 284-289

 

ISSN:0021-1915

 

年代: 1977

 

DOI:10.1080/10256017708544066

 

出版商: Taylor & Francis Group

 

关键词: antibodies;antigens;blood serum;hyperthyroidism;hypothyroidism;iodine 125;nuclear medicine;radioimmunoassay;radiopharmaccuticals;thyroid;triiodothyronine

 

数据来源: Taylor

 

摘要:

Specific method for accurate radioimmunoassay (RIA) of total triiodothyronine (T3) in unextracted human serum has been developed. The method is used for estimation of either free T3 in serum or total T3 by inhibition of the binding sites of T3 to protein using TCA/NaOH mixture. The technique used is RIA, using T3-antibodies from Amersham and dextran charcoal is used for separation of free fraction from the bind antibody antigen protein.

 

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