Ultrastructural Evidence for Gamma Aminobutyric Acid-Immunoreactive Synapses on Somatostatin-Immunoreactive Perikarya in the Periventricular Anterior Hypothalamus
作者:
John O. Willoughby,
Dimitra Beroukas,
William W. Blessing,
期刊:
Neuroendocrinology
(Karger Available online 1987)
卷期:
Volume 46,
issue 3
页码: 268-272
ISSN:0028-3835
年代: 1987
DOI:10.1159/000124830
出版商: S. Karger AG
关键词: Somatostatin;Gamma aminobutyric acid;Immunocytochemistry;Anterior hypothalamus;Growth hormone
数据来源: Karger
摘要:
Somatostatin-immunoreactive perikarya in the periventricular anterior hypothalamus were demonstrated to be surrounded by gamma aminobutyric acid GABA-immunoreactive nerve terminals, by combining pre-embedding immunocytochemistry for somatostatin and gold labelling post-embedding immunocytochemistry for GABA. Ultrastructural studies revealed that in each 100-nm section, cells immunoreactive for somatostatin (n = 62) were contacted by a mean of 7.6 + 0.4 terminal profiles of which 3.0 + 0.3 (40%) were GABA-immunoreactive. Most GABA-immunoreactive terminals contained clear rounded vesicles and, where synaptic specializations were well demonstrated, appeared to be symmetric. The finding provides evidence that there is a significant GABA input to somatostatin neurons, an observation strengthening the hypothesis that GABA may inhibit somatostatin neurons, thereby causing increased secretion of growth hormone.
点击下载:
PDF
(876KB)
返 回