Repetitive subsecond fluorescence spectra and anisotropy measurements using an optical multichannel analyzer
作者:
David A. Deranleau,
Edgar Stu¨ssi,
期刊:
Review of Scientific Instruments
(AIP Available online 1987)
卷期:
Volume 58,
issue 10
页码: 1840-1842
ISSN:0034-6748
年代: 1987
DOI:10.1063/1.1139529
出版商: AIP
数据来源: AIP
摘要:
A vidicon tube‐based optical multichannel analyzer (OMA) was interfaced to a minicomputer to facilitate the acquisition of real‐time light transmission (or fluorescence anisotropy), right‐angle scattering, and fluorescence spectra at subsecond rates. The apparatus was designed for kinetic studies of the optical responses of living cells to stimulation with physiological and other activators, and features menu‐driven interactive graphics for presentation and handling of the intensity, wavelength, and time data in two or three dimensions. Stimulus‐induced secretion of acriflavine‐loaded human blood platelet dense granule contents is shown as an example of the apparatus capabilities.
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