Quantitative Analysis of the Loop Diuretic Torasemide in Tablets and Human Urine by HPLC-EC
作者:
M. Begoña Barroso,
RosaM. Alonso,
RosaM. Jiménez,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 1996)
卷期:
Volume 19,
issue 2
页码: 179-186
ISSN:1082-6076
年代: 1996
DOI:10.1080/10826079608005504
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A high Performance Liquid Chromatographic method with amperometric detection for the determination of the loop diuretic l-Isopropyl-3-[4 - (3-methylphenylamino)-3-pyridinesulphonyl] urea, torasemide, is reported using a μ-Bondapak C18column and a mobile phase water:acetonitrile (65:35), 5 mM in potassium dihydrogenphosphate/dipotassium hydrogenphosphate at pH 5.3. Eluant is monitored at +1300 mV with an amperometric detector equipped with a glassy carbon working electrode. The method showed a determination limit of 8 ppb and a reproducibility in terms of relative standard deviation lower than 2% in intra-day assays and 5% in inter-day assays. The HPLC-EC method was applied to urine samples obtained from a healthy volunteer. Concentration levels of torasemide at different time intervals were monitorized and results were in agreement with the pharmacokinetic parameters of this diuretic. The determination of torasemide in urine required a liquid-liquid extraction prior to chromatographic analysis due to the interferences found in urine matrix. With this simple clean-up procedure, recoveries around 70% were achieved. Torasemide was directly determined in tablets after dissolution of the powder in a methanolic solution.
点击下载:
PDF (301KB)
返 回