Antigenic Determinant of Trichosanthin Peptide Fragments
作者:
PaoYi,
HuiYa,
YaoZhen,
MeiKuang,
期刊:
Immunopharmacology and Immunotoxicology
(Taylor Available online 1988)
卷期:
Volume 10,
issue 2
页码: 131-139
ISSN:0892-3973
年代: 1988
DOI:10.3109/08923978809014328
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractPurified plant protein, Trichosanthin (TCS), was partially digested with chymotrypsin to produce peptide fragments. Two large fragments, I and II, have been isolated and identified as C-terminal peptides located at sequence positions between 79–234 and 107–234, Their molecular weights determined by electro-phoretic mobility on SDS gel were 16,000 and 14,000 daltons. Studies using fluorescence quenching measurements by titrating anti-TCS Fab with TCS and fragment I and II showed that four epitopes were present in intact TCS and one each epitope in fragment I and II. One of the epitopes is therefore located between sequence positions 107 and 234.
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