Cellular Localization of Human Herpesvirus 8 in Nonneoplastic Lymphadenopathies and Chronic Interstitial Pneumonitis by in Situ Polymerase Chain Reaction Studies
作者:
Raffaella,
Trovato Mario,
Luppi Patrizia,
Barozzi Luigi,
Prato Antonio,
Maiorana Salvatore,
Lico Roberto,
Marasca Pietro,
Torricelli Giuseppe,
Torelli Luca,
期刊:
Journal of Human Virology
(OVID Available online 1999)
卷期:
Volume 2,
issue 1
页码: 38-44
ISSN:1090-9508
年代: 1999
出版商: OVID
关键词: HHV-8;KSHV;in situ PCR;benign lymphadenopathy;interstitial pneumonitis.
数据来源: OVID
摘要:
Objectives:To study the cellular localization of human herpesvirus 8 (HHV-8) in rare cases of HHV-8 infection from Italy that are associated neither with human immunodeficiency virus (HIV) infection nor Kaposi's sarcoma (KS).Methods:The presence and distribution of HHV-8-infected cells was investigated by direct in situ polymerase chain reaction (PCR) in the lymph node tissues from 2 patients with reactive lymphadenopathies with florid follicular hyperplasia and increased vascularity and in the lung tissue from 1 patient with chronic interstitial pneumonitis.Results:HHV-8 was localized in lymphoid and monocyte-macrophage cells scattered in the interfollicular regions of both lymph nodes but not in endothelial cells. In the lung tissue, HHV-8 was found in the inflammatory cells infiltrating the interalveolar interstitium, in endothelial cells of the pulmonary vasculature, and in rare pneumocytes.Conclusions:HHV-8 can infect nonneoplastic lymph nodes of immunocompetent subjects, and the distribution of infected cells outside of the germinal centers resembles that of Epstein-Barr virus (EBV)-infected cells in the lymph nodes in the course of infectious mononucleosis. Endothelial cells and pneumocytes may be a target of HHV-8 infection out of the KS setting, at least in the presence of a chronic inflammatory process.Journal of Human Virology 1999;2:38-44 © Lippincott Williams & Wilkins, Inc.
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