Parthenogenetic development of dwarf surf dam,Mulinia lateralis, oocytes treated with polar body suppressing agents
作者:
JOHN SCARPA,
KATSUHIKOT. WADA,
STANDISH ALLEN,
期刊:
Invertebrate Reproduction & Development
(Taylor Available online 1992)
卷期:
Volume 22,
issue 1-3
页码: 47-55
ISSN:0792-4259
年代: 1992
DOI:10.1080/07924259.1992.9672256
出版商: Taylor & Francis Group
关键词: parthenogenesis;Mulinia lateralis;bivalve;polar body;centrosome;meiosis;flow cytometry;polyploidy
数据来源: Taylor
摘要:
Parthenogenesis following oocyte activation has been observed in a number of marine invertebrates, but the fate of parthenogenesis in bivalve mollusc embryos is unclear. We used the dwarf surf clam,Mulinia lateralis, to examine parthenogenetic development of KC1-activated oocytes using the polar body suppressing agents caffeine and heat or cytochalasin B. Development was followed by epifluorescence microscopy and flow-cytometric analysis using the DNA-specific fluorochrome DAPI. All agents suppressed polar body formation to some degree, putatively increasing the ploidy level and retaining a meiotic centrosome in the zygote; but the zygotes failed to develop normally. Failure of the zygotes to develop suggests that the meiotic centrosome is incapable of participating in mitosis in bivalves.
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