FiftySalmonella dublinstrains isolated from cattle and human diarrhoeal cases were assayed for toxin production, haemagglutination, cell‐surface hydrophobicity and fibronectin‐binding properties. Most strains (65% of tested) produced cytotonic toxins and cytotoxic factors when tested on Chinese hamster ovary (CHO) cells and rabbit skin test. However, only three strains produced a skin‐permeability factor as determined in pig skin intra‐dermal tests. None of the strains were positive in pig intestinal loop tests. Six of the 32 strains tested for125I‐fibronectin and its125I‐29 kDa N‐terminal domain binding showed 10–17% and 6–10% binding, respectively. Most of the strains expressed mannose‐sensitive haemagglutination (MSHA) (76%) and high cell‐surface hydrophobicity (74%) when grown at 37 °C. At 20 °C the expression of MSHA and especially the expression of high cell‐surface hydrophobicity were reduced. Twelve strains grown at 37 °C did not haemagglutinate erythrocytes from five animal species used in this study, while six of these strains expressed high cell‐surface hydrophobicity.Salmonella dublinstrains isolated in Denmark appeared to express a higher frequency of fimbriae type 1 (MSHA) and a lower frequency of high cell‐surface hydrophobicity than the