The bimodally respiring catfishClarias macrocephalusGünther responded to a toxic extract ofCroton tiglium(Euphorbiaceae) seeds by increased air breathing under both normoxic (8.1 ± 0.4 mgO2l−1) and hypoxic (0.7 ± 0.1 mgO2l−1) conditions. Fish in hypoxia survived longer than those in normoxia when surface access was provided. When air breathing was prevented, survival time in toxin was greatly reduced at both levels of dissolved oxygen, and fish in normoxia survived longer than those in hypoxia. Non‐toxin controls without surface access survived in normoxia but in hypoxia died at the same time as the fish in toxin. These results suggest that air breathing increases the resistance offish to toxins by permitting a decrease in the rate of gill ventilation and hence the rate at which toxins are