The primary antibody response of malaria patients to Plasmodium falciparum sexual stage antigens which are potential transmission blocking vaccine candidates
作者:
C.S.L. ONG,
K.Y. ZHANG,
S.J. EIDA,
P.M. GRAVES,
C. DOW,
M. LOOKER,
N.C. ROGERS,
P.L. CHIODINI,
G.A.T. TARGETT,
期刊:
Parasite Immunology
(WILEY Available online 1990)
卷期:
Volume 12,
issue 4‐5
页码: 447-456
ISSN:0141-9838
年代: 1990
DOI:10.1111/j.1365-3024.1990.tb00980.x
出版商: Blackwell Publishing Ltd
关键词: malaria;Plasmodium falciparum;sexual stage antigens;transmission‐blocking;antibodies
数据来源: WILEY
摘要:
SummaryThirty serum samples collected from adult patients attending the Hospital for Tropical Diseases, London, withP. falciparummalaria, were studied. Sera were screened by indirect immunofluorescence for anti‐gametocyte antibodies. Twelve of the serum samples taken from 14 patients with primary infections were found to have both IgM and IgG antibodies to gametocyte antigens and total Ig titres comparable with those of patients who had had previous malaria attacks. Sera of individuals from hyperendemic areas have been found to immunoprecipitate the 230 and 48/45 kD gametocyte surface antigens which are known targets of transmission blocking antibodies. To investigate the epitope specificity of the serum samples from our adult patients, competitive ELISAs with 3 monoclonal antibodies (MAbs) that block transmission and recognize different epitopes on the 48/45 Kd antigen, were carried out. Specific antibodies for these epitopes were found in 60% of the sera while nearly a third were able to inhibit the binding of at least two MAb
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