Differentiation of Biologically Distinct Cucumber Mosaic Virus Isolates by PAGE of Double-Stranded RNA
作者:
R.D. Pares,
M.R. Gillings,
L.V. Gunn,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 34,
issue 1
页码: 23-29
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150259
出版商: S. Karger AG
关键词: Cucumber mosaic virus;dsRNA analysis;Isolate differentiation
数据来源: Karger
摘要:
Electrophoresis on 5% polyacrylamide was used to analyze dsRNAs of 26 cucumber mosaic virus isolates propagated in Nicoîiana tabacum. There was variation between isolates in the migration of each of the dsRNAs 1,2, and 3. Comparison of the dsRNA profiles enabled each isolate to be allocated to 1 of 7 distinct dsRNA profile types. Two distinct and readily distinguishable isolates were mixed in planta and dsRNA from these infected plants compared with in vitro mixtures of them. All bands from both types were present, indicating that the differences were real and reproducible. This method is of value as a means of classifying cucumber mosaic virus isolates, as it more closely reflects a range of biological characteristics than do other methods currently used
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