COMPARISON OF LYSOZYME ADSORPTION TO IMMOBILIZED CIBACRON BLUE 3GA USING VARIOUS MEMBRANE SUPPORTS
作者:
Shing-Yi Suen,
Rui-Long Chen,
Yi-Da Tsai,
期刊:
Journal of Liquid Chromatography & Related Technologies
(Taylor Available online 2000)
卷期:
Volume 23,
issue 2
页码: 223-239
ISSN:1082-6076
年代: 2000
DOI:10.1081/JLC-100101447
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Lysozyme adsorption to immobilized Cibacron Blue 3GA using different membrane supports, regenerated cellulose membrane discs, and polysulfone hollow fibers, is investigated in this study. The relevant adsorption properties using traditional chromatographic beads were also measured for comparison. The experimental results demonstrated distinct adsorption properties for different solid supports. The membrane discs possessed the best thermal resistance among the three adsorbents assessed, which essentially results from their higher thermal stability. Moreover, the saturation capacity of lysozyme onto membrane discs was found close to the ligand density for gel beads, whereas the hollow fibers were of much greater saturation capacity.
点击下载:
PDF (285KB)
返 回