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A Rapid Scanning Dual Wavelength Spectrophotometer

 

作者: Britton Chance,   Norman Graham,  

 

期刊: Review of Scientific Instruments  (AIP Available online 1971)
卷期: Volume 42, issue 7  

页码: 941-945

 

ISSN:0034-6748

 

年代: 1971

 

DOI:10.1063/1.1685311

 

出版商: AIP

 

数据来源: AIP

 

摘要:

The dual wavelength spectrophotometer, noted for its high sensitivity and accuracy in the recording of oxidation‐reduction and other reactions of membrane bound components in turbid suspensions of biological materials, has now been modified to scan the spectrum of these components over a reasonable wavelength range (40 nm) and in a short time (rates as rapid as 20 nm/sec). The baseline precision is not remarkable (approximately 1% error) but the method retains the intrinsic noise rejection properties of the dual wavelength technique. Two forms of the apparatus are discussed, one a simple modification involving a previously described time sharing compensating circuit, and the other incorporating a tapped potentiometer for more precisely correcting the baseline, and a comparator and error detector for correcting wavelength dependent sensitivity error.

 

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