Molecular Cloning and Partial Characterization of a Parrot Papillomavirus
作者:
Kerry O’Banion,
Elliott R. Jacobson,
John P. Sundberg,
期刊:
Intervirology
(Karger Available online 1992)
卷期:
Volume 33,
issue 2
页码: 91-96
ISSN:0300-5526
年代: 1992
DOI:10.1159/000150236
出版商: S. Karger AG
关键词: Parrot papillomavirus;Molecular cloning;Hybridization;DNA sequencing
数据来源: Karger
摘要:
The genome of a papillomavirus isolated from a cutaneous lesion on the head of an African grey parrot (Psittacus erithacus timneh) was cloned into pBR322, and a restriction map was prepared. Several short portions of the DNA were sequenced allowing the genome to be aligned with HPV-1a. In Southern blot hybridizations, under conditions of low and medium stringency (Tm–40and –33 °), but not at a higher stringency, this viral DNA annealed weakly with only 1 of 17 mammalian papillomavirus genomes tested. Furthermore, PePV DNA hybridized with the DNA from the European chaffinch only at low stringency, indicating that it represents a unique avian papillomav
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