MayfliesTimpanoga hecuba, caddisfliesDicosmoecus gilvies, mosquitoesCulex peusand midgesCricotopussp. were tested in the laboratory to determine the effects of air-supersaturated water on survival and adult emergence. The acute 96-hour LC50 value (lethal concentration which killed 50% after 96 hr) for mayflies was 128.9% saturation. Times to death and mean times to 50% death were determined. Adult midges and mosquitoes emerged at 141% and 143%, respectively. Good survival and emergence occurred in controls for all test groups. Adult mayflies did not successfully emerge or survive at concentrations of ≥135%. Adult caddisflies did not emerge at concentrations of ≥134%. Bubbles which were observed in body fluids caused buoyancy problems and raised the insects to the water surface. Data indicate that mosquitoes were highly tolerant and midges were moderately tolerant of air-supersaturated water. Mayflies and caddisflies were relatively sensitive. All insects were more tolerant of air-supersaturated water than fish species.