Immunogold Labeling of Alzheimer Paired Helical Filaments with Ganglioside MAB A2B5
作者:
Jane Clements,
Alvin Beitz,
Carolyn Emory,
William Frey,
期刊:
Alzheimer Disease and Associated Disorders
(OVID Available online 1990)
卷期:
Volume 4,
issue 1
页码: 35-42
ISSN:0893-0341
年代: 1990
出版商: OVID
关键词: Neurofibrillary tangles;Ultrastructure;Electron microscopy;A2B5.
数据来源: OVID
摘要:
The ganglioside monoclonal antibody A2B5 has previously been used at the light microscopic level to label Alzhimer neurofibrillary tangles (NFTs). Light microscopic analysis, however, could not reveal whether the A2B5 antibody-labeled NFTs or membrane fragments associated with NFTs. Therefore, we used pre-embedding immunohistotochemical electron microscopy to examine A2B5 labeling of NFT. We found that the A2B5 antibody does indeed label a NFT antigen associated with the paired helical filament (PHF) structure, while no significant labeling of membranes or membrane fragments was observed. However, no clear periodicity of the immunogold label on the PHF was found.
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