Of 289 fungi and actinomycetes tested, only 20 were found to produce high yields of proteolytic enzymes when grown in submerged culture. Strains ofGliocladium roseumandAlternaria tenuiswere found to be consistently proteolytic, and other good strains were also found in the generaChaetomium,Sordaria,Epicoccum,Rhizoctonia, andStreptomyces.