Cytology ofViciaspecies. IX. Nuclear DNA amount, chromatin organization and computer aided karyotyping of a Russian accession ofVicia faba.L
作者:
V.V Kotseruba,
G. Venora,
S. Blangiforti,
M.Ruffini castiglione,
R. cremonini,
期刊:
Caryologia
(Taylor Available online 2000)
卷期:
Volume 53,
issue 3-4
页码: 195-204
ISSN:0008-7114
年代: 2000
DOI:10.1080/00087114.2000.10589196
出版商: Taylor & Francis Group
关键词: chromatin organization;chromosome banding;image analysis;plant chromosomes;Vicia faba
数据来源: Taylor
摘要:
The Faba section of theViciagenus, which includes several widely distributed species in Mediterranean countries is considered the most evolved. Indeed, it is known nuclear DNA values range from 9.15 pg. (2C value) inVicia bithynica, to 26.56 pg. (2C value) in Vicia faba which has the highest DNA content in the genus. Banding pattern of metaphase chromosomes and nuclear DNA content in root meristematic cells of a Russian accession ofV. fabawere determined.Microdensitometric evaluation of nuclear absorption at different thresholds of optical density after Feulgen reaction indicated the organisation of the chromatin in-terphase nuclei. Karyotype, morphometric data and density distribution along the mid-rib of a chromatid were employed for the construction of a quantitative chromosome map generated by an automated image analysis system. The classification of Stebbins(1971), the TF%% values (Huziwara 1962) and Rec. and Syi indices (Greilhuber and Speta 1976) were used to perform the analysis. The results are compared with previous data concerningV. faba.
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