Improved Ultraviolet Microbeam Apparatus
作者:
Robert B. Uretz,
Robert P. Perry,
期刊:
Review of Scientific Instruments
(AIP Available online 1957)
卷期:
Volume 28,
issue 11
页码: 861-866
ISSN:0034-6748
年代: 1957
DOI:10.1063/1.1715750
出版商: AIP
数据来源: AIP
摘要:
An ultraviolet microbeam apparatus utilizing the principle of incident illumination has been developed for the irradiation of small parts of living cells or other microscopic objects. A reflecting microscope objective is used simultaneously in a visible light viewing‐aiming system and in a bombardment system to focus a spot of ultraviolet light (as small as one micron in diameter) onto part of an experimental preparation. Provision is made in the apparatus for auxiliary phase contrast viewing and for monitoring of the energy in the microbeam. Details of construction and alignment are given and the distribution of intensities in the microbeam is discussed.
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