Flow kinetics ofL‐asparaginase attached to nylon tubing
作者:
Peter S. Bunting,
Keith J. Laidler,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1974)
卷期:
Volume 16,
issue 1
页码: 119-134
ISSN:0006-3592
年代: 1974
DOI:10.1002/bit.260160109
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractL‐Asparaginase has been attached by chemical means to the inner surface of nylon tubing. An experimental study has been carried out of the flow kinetics for such a system, asparagine solutions at various concentrations being passed through two lengths of tubing at various flow rates. Measurements were made of the concentration of the product ammonia at the tube exit, and of the rate of formation of ammonia, under the various conditions. Apparent Michaelis constants,Km(app), were some three orders of magnitude higher than theKmfor the enzyme in free solution (∼13 × 10−6JM). The results were analyzed with respect to the theoretical treatment described in the preceding paper (Kobayashi and Laidler), three different methods being employed. It is concluded that at lower substrate concentrations and flow rates the reactions are largely diffusion‐controlled, the enhancedKm(app) values being largely if not entirely due to the diffusion control; ionic strength studies showed electrostatic repulsion effects to be unimportant. At high concentrations and high flow rates (when the diffusion layer is of negligible thickness) the diffusional effects are minimized, andKm(app) approaches the trueKmvalue for the immobilize
点击下载:
PDF
(723KB)
返 回