Mapping a marsupial X chromosome using kangaroo-mouse somatic cell hybrids
作者:
J.A. Donald,
R.M. Hope,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1981)
卷期:
Volume 29,
issue 3
页码: 127-137
ISSN:1424-8581
年代: 1981
DOI:10.1159/000131561
出版商: S. Karger AG
数据来源: Karger
摘要:
A series of marsupial-eutherian somatic cell hybrids was produced by fusion between lymphocytes from the red kangaroo (Macropus rufus) and HPRT-deficient mouse cells. The hybrids lost marsupial chromosomes and could therefore be used to map marsupial genes. Several of the hybrids contained a complete red kangaroo X chromosome and expressed the kangaroo form of the enzymes HPRT, G6PD, and PGKA. A number of HPRT-deficient revertant cell lines were derived from the hybrids. These possessed a variety of partially deleted X chromosomes. With these cell lines, it has been possible to establish the X-linkage of the genes for HPRT, G6PD, and PGKA in this marsupial and to localize these three genes to the terminal portion of the euchromatic arm of the red kangaroo X chromosome.
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