Immunogold detection of glycoprotein antigens in sea urchin embryos
作者:
Nancy C. Benson,
Stephen C. Benson,
Fred Wilt,
期刊:
American Journal of Anatomy
(WILEY Available online 1989)
卷期:
Volume 185,
issue 2‐3
页码: 177-182
ISSN:0002-9106
年代: 1989
DOI:10.1002/aja.1001850210
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractFour developmental stages of sea urchin embryos were labeled with colloidal gold in an attempt to elucidate the intracellular trafficking patterns within the cells that produce the glycoprotein matrix of the embryonic spicule. The primary mesenchyme cells (PMCs) form a syncytium and secrete an organic matrix on which calcium carbonate is laid down to form an endoskeletal spicule. The organic matrix has been isolated and characterized as glycoprotein consisting of four major bands. Polyclonal antibodies to these glycoproteins were used to label embryos from the mesenchyme blastula, early gastrula, late gastrula, and plutei stages of development. The label is concentrated in the Golgi complex and associated vesicles, in secretory vesicles, and in the organic matrix. The density of the labeling increases as development proceeds.
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