Uv resonance Raman spectroscopy of nucleic acid duplexes containing A‐U and A‐T base pairs
作者:
Christine A. Grygon,
Thomas G. Spiro,
期刊:
Biopolymers
(WILEY Available online 1990)
卷期:
Volume 29,
issue 4‐5
页码: 707-715
ISSN:0006-3525
年代: 1990
DOI:10.1002/bip.360290405
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractResonance Raman spectra have been obtained at several uv wavelengths (200–266 nm) for poly(rA)‐poly(rU), poly(dA‐dU), poly(dA)‐poly(dT), and poly(dA‐dT), representing nucleic acid duplexes containing A‐U and A‐T base pairs with different stacking interactions and different backbone conformations. Frequency shifts are seen in the exocyclic modes corresponding to coupled C4O and C5C6 stretching of U and T, although the NH2scissors frequency of A is unshifted relative to that of the mononucleotide. These frequency patterns are interpreted as the superposition of H bonding and dipole–dipole coupling effects. Strong hypochromism is seen for most of the ring modes, resulting from the absorption hypochromism and from shifts in the electronic transitions due to stacking. The effects are larger for A bands in the homopolymers than in the heteropolymer duplexes, reflecting the larger influence of A/A than A/U(T) stacking. Poly(rA)‐poly(rU) stands out among these polymers in showing 10 cm−1downshifts in one U and several A modes. These shifts may be related to the A form structural parameters of this duplex, but the physical m
点击下载:
PDF
(626KB)
返 回