In this paper, the study on the effect of cAMP on erythrocyte membrane proteins by FTIR, deconvolution and curvefitting was reported. It was found that cAMP affects the secondary structure of membrane proteins by changing random and β-turn regions to the α-helix segments. The regulation of cAMP has a best concentration region, during which cAMP has the strongest regulating function. Meanwhile, cGMP and ATP has a negative effect on membrane proteins' secondary structure comparing to cAMP.