Poly(methoxygalacturonide) lyase immobilized via titanium onto solid supports
作者:
W. H. Hanisch,
Pamela A. D. Rickard,
S. Nyo,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1978)
卷期:
Volume 20,
issue 1
页码: 95-106
ISSN:0006-3592
年代: 1978
DOI:10.1002/bit.260200108
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractPoly(methoxygalacturonide) lyase (PMGL) (E.C. 4.2.2.10) was purified from a commercial preparation and immobilized by the metal link method. The properties of DEAE‐cellulose‐Ti‐PMGL and of porous glass‐Ti‐PMGL were compared with those of the native enzyme; despite the presence of the metal and the heterogeneity of the substrate, pectin, typical substrate–enzyme‐support interactions were demonstrated by shifts in pH optima andKMvalues. The possible industrial application of DEAE‐cellulose‐Ti
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