Assignment of the uteroferrin gene (ACP5) to swine chromosome 2q12→q21 by fluorescence in situ hybridization
作者:
H. Yasue,
H. Kusumoto,
H. Mikami,
期刊:
Cytogenetic and Genome Research
(Karger Available online 1995)
卷期:
Volume 71,
issue 3
页码: 249-252
ISSN:1424-8581
年代: 1995
DOI:10.1159/000134120
出版商: S. Karger AG
数据来源: Karger
摘要:
A cosmid clone containing the uteroferrin gene (ACP5) was selected from a cosmid library of swine genomic fragments by colony hybridization using uteroferrin cDNA as a probe. The genomic fragment thus cloned was examined by Southern blot and sequence analysis which demonstrated that it contained at least a part of the uteroferrin gene. The cosmid clone DNA was labeled with biotin, and used as a probe for in situ hybridization to swine chromosomes. Hybridization was visualized by the FITC-labeled streptavidin/biotinylated anti-streptavidin system together with R-banding of chromosomes. The hybridization signals revealed that the uteroferrin gene (ACP5) is located on swine chromosome 2q12→q2
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