Chromatographic Purification of Tritiated Steroids Prior to Use in Radioimmunoassay
作者:
FredesmindaS. Manlimos,
GuyE. Abraham,
期刊:
Analytical Letters
(Taylor Available online 1975)
卷期:
Volume 8,
issue 6
页码: 403-410
ISSN:0003-2719
年代: 1975
DOI:10.1080/00032717508058223
出版商: Taylor & Francis Group
关键词: Gas chromatography;steroids;radioimmunoassay;purification
数据来源: Taylor
摘要:
The purity of tritiated steroids used as reagents in radio immunoassay plays an important role in the reliability of the assay. These radioactive reagents should be assessed for purity upon receipt and the purity should be checked periodically afterward. For such purposes, we have used chromatographic purification on Celite microcolumns. By charging the polarity of the stationary and mobile phases, 20 different tritiated steroids with a wide range of polarity could be purified on these microcolumns. This approach is easy, rapid, economical, and reliable.
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