Communication: Synthesis of a Library of β-GlcNAc Glycosides to Screen for Efficient in Vivo Glycosyltransferase Acceptors
作者:
Yili Ding,
Yoshiaki Miura,
James R. Etchison,
Hudson H. Freeze,
Ole Hindsgaul,
期刊:
Journal of Carbohydrate Chemistry
(Taylor Available online 1999)
卷期:
Volume 18,
issue 4
页码: 471-475
ISSN:0732-8303
年代: 1999
DOI:10.1080/07328309908544011
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Artificial glycosides are used to prime the biosynthesis of glycosaminoglycan chains and those typical ofN- andO-linked oligosaccharides, e.g.,N-acetyllactosamine repeats.1-8Several studies have shown that the aglycon influences the amount and type of products assembled on such glycosides.2-6The amount of product made depends on two factors. The first is the ability of the glycoside to penetrate the plasma membrane and the Golgi membrane where the glycosyltransferases are located. The second is the structure of the aglycon since it can influence the addition of the first monosaccharide as well as extension with additional residues. In some instances, the aglycon is thought to mimic a portion of an underlying polypeptide chain.2-4,6The choice of aglycons is important, but there are no guidelines to direct the choice since many factors such as their stability to glycosidases, membrane-permeability, cellular toxicity or limitation of specificity and affinity can all be important. In the absence of such guidelines, we streamlined the process of aglycon evaluation and report here an efficient strategy to synthesize GlcNAcβ-R libraries with various aglycons.
点击下载:
PDF (251KB)
返 回