A Method for the Identification ofStreptococcus mutansin Gingival Margin Plaque by Immunofluorescence
作者:
M.S. Bush,
S.J. Challacombe,
H.N. Newman,
期刊:
Caries Research
(Karger Available online 1990)
卷期:
Volume 24,
issue 1
页码: 23-29
ISSN:0008-6568
年代: 1990
DOI:10.1159/000261232
出版商: S. Karger AG
关键词: Caries;Immunofluorescence;S. mutans
数据来源: Karger
摘要:
A method was developed to identify Streptococcus mutans in natural dental plaque by indirect immunofluorescence staining, using a high-titred polyclonal antiserum raised against a serotype c strain of S. mutans followed by an FITC conjugate. Specificity was determined by staining 45 representative strains of plaque organisms, which demonstrated minimal cross-reactions. In vitro incubation of S. mutans NCTC 10449 films with a human serum containing antibodies to S. mutans and the presence of extracellular polysaccharide did not inhibit staining. The staining method enabled 98% of the streptococci to be detected in mixtures of S. mutans NCTC 10449 and Lactobacillus casei NCTC 10302. S. mutans was detected at a ratio of 1:100,000 in mixtures of pure cultures. In plaque samples, S. mutans could be distinguished from other organisms, including an unidentified cross-reacting bacillus found in some gingival plaque samples. The results suggest that immunofluorescence is a fast, practical method for identifying specific bacteria in plaque and, therefore, could be of use in microbiological studies of caries.
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