The Effect of Tumor Burden on Ornithine Decarboxylase Activity in Mice
作者:
SaydjariRami,
AlexanderRobert W.,
UppJames R.,
PostonGraeme J.,
BarrancoSam C.,
TownsendCourtney M.,
ThompsonJames C.,
期刊:
Cancer Investigation
(Taylor Available online 1991)
卷期:
Volume 9,
issue 4
页码: 415-419
ISSN:0735-7907
年代: 1991
DOI:10.3109/07357909109084639
出版商: Taylor&Francis
数据来源: Taylor
摘要:
AbstractPolyamines are essential for cell growth of normal and neoplastic tissue.α-Difluoromethylornithine (DFMO) is a known irreversible inhibitor or ornithine decarboxylase (ODC), the rate-limiting enzyme in polyamine biosynthesis. The purpose of this study was to examine the effects of tumor burden on ODC in tissues of tumorbearing compared with tumor-free mice. Twenty-eight male Balb/c mice were divided into four groups of 7 each. Groups 1 and 2 were inoculated subcutaneously with 10 X 106MC-26 mouse colon adenocarcinoma cells. Groups 3 and 4 were kept as tumor-free controls. Ten days after inoculation, groups 2 and 4 were injected with DFMO (200 mg/kg) intraperitoneally (IP) while Groups 1 and 3 received saline. Two hours after the injection of DFMO the animals were sacrificed. The tumor, pancreas, kidney, and liver were excised and analyzed for ODC activity. DFMO caused a significant reduction (compared with controls that did not receive DFMO) in the ODC activity of tumors; however, ODC activity of the kidney, pancreas, and liver of tumor-bearing mice was not affected. Additionally, the basal ODC activity in the kidney, liver, and pancreas of tumor-bearing mice was significantly lower compared with tumor-free controls. DFMO lowered ODC activity in the kidney, pancreas, and liver of tumor-free mice. These results suggest that the presence of MC-26 tumor causes systemic effects that alter ODC activity and the response to a known inhibitor of ODC.
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