Quantitative Measurement of Fluorescent Intensity of the Rat Liver Surface Using Video Fluorescence Laparoscopy Following Intravenous Fluorescein Injection
作者:
Hiroto WAKABAYASHI,
Haruhide SHINZAWA,
Hirotaka TODA,
Ichiro AOYAMA,
Makoto KUBOKI,
Masamichi ISHIBASHI,
Takahumi SAITOH,
Katsuaki UKAI,
Nobuo YAMADA,
Hitoshi TOGASHI,
Tsuneo TAKAHASHI,
Makoto ISHIKAWA,
期刊:
Digestive Endoscopy
(WILEY Available online 1992)
卷期:
Volume 4,
issue 1
页码: 3-7
ISSN:0915-5635
年代: 1992
DOI:10.1111/j.1443-1661.1992.tb00220.x
出版商: Blackwell Publishing Ltd
关键词: laparoscopy;fluorescence laparoscopy;CCD camera;fluorescein;quantitative analysis
数据来源: WILEY
摘要:
Abstract:A new method for the quantitative measurement of fluorescent intensity of the rat's liver surface after fluorescein injection is described. The measuring system of fluorescent intensity on the rat liver surface consisted of a video laparoscopy with a video densitometer. An excitation filter was placed in the light source and an absorbing filter was attached to the eyepiece of the magnifying scope in this fluorescence video laparoscopy system. Fluorescein was injected intravenously into the inferior vena cava. Video fluorescence images of the liver surface were recorded with a CCD TV camera which was connected to the scope. In measuring the mean intensity of video images, a region of interest was designated on the TV monitor. The fluorescent intensity in this region was measured by a video densitometer and expressed in volts. The time‐intensity curve was graphically presented using a pen recorder. In this way, the time course of the liver surface fluorescent intensity could be analyzed with data expressed in volts. In normal livers, the fluorescent intensity began to rise 4.0 ± 0.5 seconds after fluorescein injection and reached a peak at 14.4 ± 1.5 seconds. Thereafter, the intensity fell slightly but then rose again to a second peak 170±50 seconds after injec
点击下载:
PDF
(558KB)
返 回