Instability of actinomadurae protoplasts generated by lytic enzyme treatment
作者:
D.G. Allison,
P.M. Roberts,
期刊:
Letters in Applied Microbiology
(WILEY Available online 1989)
卷期:
Volume 8,
issue 1
页码: 1-3
ISSN:0266-8254
年代: 1989
DOI:10.1111/j.1472-765X.1989.tb00210.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
The effect of lytic enzyme treatment upon protoplast formation and reversion in three species ofActinomadurahas been determined. Incubation in the presence of lytic enzyme L2 generates large protoplasts (4 μm diameter) which remain intact for only 2h. In comparison, protoplasts formed by the degradative effects of the enzymes lysozyme and L1 are smaller (2 μm diameter), and remain stable for up to 18h. This results in a greater efficiency of regeneration. Lytic enzyme L2 has been shown to contain impurities, including proteolytic activity, which may affect cell wall regeneratio
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