Cytotoxic effects of benzene on mouse germ cells determined by flow cytometry
作者:
M. Spanò,
F. Pacchierotti,
R. Uccelli,
R. Amendola,
C. Bartoleschi,
期刊:
Journal of Toxicology and Environmental Health
(Taylor Available online 1989)
卷期:
Volume 26,
issue 3
页码: 361-372
ISSN:0098-4108
年代: 1989
DOI:10.1080/15287398909531259
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
Flow cytometric (FCM) DNA content measurements were performed on testicular monocellular suspensions obtained from mice exposed per os to 0, 1, 2, 4, 6, and 7 ml/kg body weight of benzene in order to investigate its cytotoxic action on germ cells. The effects of benzene were measured 7, 14, 21, 28, and 70 d after treatment. Benzene had no effect on testis weight, but FCM analysis showed the relative percentages of some cell subpopulations (tetraploid and haploid cells) to be different from the control pattern, indicating the occurrence of some cytotoxic damage to differentiating spermatogonia. These data demonstrate that spermatogenesis is sensitive to benzene single exposures as evidenced by an altered cell ratio of testicular cell types.
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