A Monoclonal Antibody which Inhibits Growth of T Cell Lines
作者:
NazareaMartina,
SaitoYuji,
OkazakiSaeko,
SuzukiNoboru,
HonjoTasuku,
期刊:
Growth Factors
(Taylor Available online 1993)
卷期:
Volume 9,
issue 2
页码: 139-147
ISSN:0897-7194
年代: 1993
DOI:10.3109/08977199309010829
出版商: Taylor&Francis
关键词: IL-2-association molecule;T cell growth inhibition;FACS profile of cell lines
数据来源: Taylor
摘要:
AbstractIn an attempt to elucidate the intricate structure and possibly the signal transduction pathway of the interleukin-2 receptor (IL-2R) we tried to produce monoclonal antibodies against putative human IL-2R-associated molecules which precipitated from YT2C2 cells with a monoclonal antibody (Mikβ3) against the human IL-2Rβchain. One of the antibodies obtained (7xA10) recognizes a cell surface molecule of about 120 kDa (p120). Cross-linking of [125I]-IL-2 and immunoprecipitation with 7xA10 suggest that the p120 protein may somehow associate physically with the IL-2Rβchain. Although p120 itself did not bind IL-2, and 7xA10 did not inhibit IL-2 binding to the IL-2R, growth of the human IL-2-dependent cell lines ED40515 and Kit225 was completely inhibited by adding 7xA10 to the culture at a relatively low concentration (0.2g/ml). At a higher concentration (2g/ml) 7xA10 inhibited the growth of YTC3 and YT2C2 cells expressing IL-2Rs with high and intermediate affinities, respectively. The B cell line FLEB-14 and the erythroid cell line K562, which both express p120 as determined by flow cytometry, but no IL-2Rαorβchains showed only slight proliferation changes at high 7xA10 concentrations. Out of 16 lymphocyte cell lines tested so far, only Molt-4, Raji and Daudi did not express p120, and therefore 7xA10 did not influence their growth behavior. p120 might be a new IL-2R association molecule which seems to play a certain role in the growth regulation of various lymphoid cell lines.
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