Reduced sedimentation equilibrium time by two‐step initial loading
作者:
G. Peter Todd,
Rudy H. Haschemeyer,
期刊:
Biopolymers
(WILEY Available online 1982)
卷期:
Volume 21,
issue 1
页码: 17-24
ISSN:0006-3525
年代: 1982
DOI:10.1002/bip.360210103
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractComputer simulation shows that the time required to attain near sedimentation equilbrium is dramatically reduced by a two‐step initial loading in which a macromolecular solution at low or zero concentration is layered above one at a higher concentration. To achieve the minimum time requires a good estimate of the molecular weight, but at least a 50% reduction in time can be achieved if the molecular weight of the macromolecule is known only within a factor of 2. Numerical solutions to the differential equation of the ultracentrifuge are calculated using the finite element method. An efficient Gaussian elimination algorithm can be used to minimize calculation time and computer storage requirement
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