Vacuum ultraviolet absorption spectra of sublimed films of nucleic acid bases
作者:
Takenori Yamada,
Hideo Fukutome,
期刊:
Biopolymers
(WILEY Available online 1968)
卷期:
Volume 6,
issue 1
页码: 43-54
ISSN:0006-3525
年代: 1968
DOI:10.1002/bip.1968.360060104
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractMeasurements of the optical absorption spectra of the sublimed films of adenine, guanine, cytosine, thymine, and uracil were extended down to 120 mμ at room temperature. Several remarkable absorption peaks were found to exist below 190 mμ in addition to the already known ones near 260 mμ and 200 mμ. The intensities of these peaks were comparable to or, in some cases, even larger than those near 260 mμ and 200 mμ. It was found that the relative intensities of the absorption peaks differed considerably from sample to sample for all five bases, probably due to variation in the arrangement of the bases in the sublimed films. It was found also that the absorption spectra of the sublimed films change with aging on standingin vacuoat room temperature. On comparing the spectra of several fresh samples it was found that the intensities of the transitions with wavelength longer than 160 mμ of adenine, guanine, and uracil films are inversely correlated with those of the transitions shorter than
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