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Assay of Sodium and Potassium Activated Adenosine Triphosphatase in Submicrogram Fragments of Renal Tubules

 

作者: WalterJ. Czaczkes,   GeraldG. Vurek,   Maurice Burg,  

 

期刊: Analytical Letters  （Taylor Available online 1979）
卷期: Volume 12, issue 8  

页码: 893-904

 

ISSN:0003-2719

 

年代: 1979

 

DOI:10.1080/00032717908059769

 

出版商: Taylor & Francis Group

 

关键词: fluorimetry;instrumentation;Na-K-ATP-ase;renal tubule

 

数据来源: Taylor

 

摘要:

An improved method for measuring Na, K-ATPase in submicrogram fragments of single renal tubules approximately one millimeter long is described. The activity is determined by coupling ATP hydrolysis stoichiometrically to pyruvate kinase and the oxidation of NADH by lactic dehydrogenase. NADH oxidation is followed fluorimetrically using an instrument specially modified for increased sensitivity and stability.

 

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