An helicoidal structure surrounding the cilium axoneme: Visualization by the monoclonal antibody CC‐248
作者:
Guillermina Bautista‐Harris,
Catherine Klotz,
Nicole Bordes,
Daniel Sandoz,
期刊:
Biology of the Cell
(WILEY Available online 1991)
卷期:
Volume 71,
issue 1‐2
页码: 191-200
ISSN:0248-4900
年代: 1991
DOI:10.1016/0248-4900(91)90065-U
出版商: Blackwell Publishing Ltd
关键词: axoneme;ciliated cells;differential labelling;monoclonal antibody
数据来源: WILEY
摘要:
Summary—Monoclonal antibody CC‐248 labels cilia differentially on Triton X‐100 permeabilized ciliated epithelium of quail oviduct by indirect immunofluorescence. On isolated ciliated cells, a punctuated staining is seen at the distal region over the bend of cilia. Electron micrographs of immunoperoxidase and immunogold techniques showed that the punctuated fluorescence corresponds to a helical disposition of CC‐248 antigenic sites. This labeling was arranged on the axonemal distal region either as a simple or a double helix externally disposed around the nine microtubular doublets. These results suggest the existence of a detergent insoluble structure in the ciliary matrix that might concern the ciliary skeleton, probably acting as an elastic recoil that keeps the structural integrity of the axoneme during bending. The cross‐reactivity of CC‐248 MAb with the intermediate filament cytoskeleton of ciliated and smooth muscle cells indicates that this structure might be related to the intermediate fila
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