Effect of Syngeneic Islet Antigen Administration on Complement-Dependent Antibody-Mediated Cytotoxicity to Islet Cells and Diabetes Onset in Diabetes-Prone Bb/Ok Rats
作者:
SchröderD.,
SchmidtS.,
KlötingIngrid,
HehmkeB.,
期刊:
Autoimmunity
(Taylor Available online 1993)
卷期:
Volume 14,
issue 4
页码: 283-289
ISSN:0891-6934
年代: 1993
DOI:10.3109/08916939309079230
出版商: Taylor&Francis
关键词: BB rat;islet antigen administration;complement-dependent antibody-mediated cytotoxicity;islet cells
数据来源: Taylor
摘要:
Diabetes-prone BB/OK rats aged 30 to 35 days were subjected to three sequential intrasplenic injections of unfractionated homogenate prepared from Langerhans' islets of newborn syngeneic BB/OK rats. Syngeneic islet antigen administration resulted in increased complement-dependent antibody-mediated cytotoxicity (C'AMC) to rat pancreatic islet cells in serum, compared to buffer-treated control animals as detected by51Cr-release assay. However, the increase of anti-islet-cell cyto toxicity neither impaired glucose tolerance nor affected the incidence of diabetes and the age at manifestation. In contrast to BB/OK rats developing diabetes, animals remaining long-term normoglycaemic did not show an enhancement of cytotoxicity to islet cells within twelve days after the first islet antigen injection as revealed retrospectively.In conclusion, humoral mediated anti-islet-cell cytotoxicity is not decisively involved in pancreaticβ-cell destruction and promotion of diabetes development in BB/OK rats, but animals becoming diabetic seem to be characterized by a stronger immunological reactivity upon syngeneic islet antigen challenge as indicated by an increase of anti-islet C'AMC compared to long-term normoglycaemic rats.
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