Restriction endonuclease banding and digestion resistant heterochromatin in triatomines, genusPanstrongylus
作者:
Ester Tartarotti,
MariaTercilia Vilela De Azeredo-Oliveira,
期刊:
Caryologia
(Taylor Available online 2000)
卷期:
Volume 53,
issue 3-4
页码: 235-243
ISSN:0008-7114
年代: 2000
DOI:10.1080/00087114.2000.10589201
出版商: Taylor & Francis Group
关键词: C-band;cytogenetic;restriction endonucleases;spermatogenesis;triatomines
数据来源: Taylor
摘要:
The present work realized a comparative study in meiosis of two triatomines,Panstrongylus herreri and P. megistus, by cytogenetic techniques involving the restriction endonucleases Hae III andAluI and C-banding. The system of sex chromosomes inPanstrongylusis of the XXX2Y type, and experiments corroborated the common origin hypothesis of the X chromosomes by fragmentation of single X. In both species the restriction endonucleases (RE) presented banding patterns in part similar to C-banding. However, in some early meiotic phases it was possible to verify differentiation of the heterochromatic pattern. This work sug-' gests that other elements besides presence of recognition sites, such as chromatin packing degree and DNAprotein interaction, act in RE results, since digestion patterns occur in early spermatogenesis. However, metaphase chromosomes were practically inaccessible to the endonucleases.
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