Monoclonal Antibody-Based Immunolocalization of Bound Triazine Residues in Two Aquatic Macrophytes (Elodea CanadensisandMyriophyllum Spicatum)
作者:
T. Giersch,
G. Sohn,
B. Hock,
期刊:
Analytical Letters
(Taylor Available online 1993)
卷期:
Volume 26,
issue 9
页码: 1831-1845
ISSN:0003-2719
年代: 1993
DOI:10.1080/00032719308017434
出版商: Taylor & Francis Group
关键词: Herbicides;atrazine;immunolocalization;monoclonal antibodies;bound residues
数据来源: Taylor
摘要:
Immunohistochemical localization offers a fast and reliable method of obtaining information about the distribution of bound pesticide residues in plants. In the present study aquatic macrophytes (Elodea canadensis, Myriophyllum spicatum) were grown in laboratory model ecosystems spiked with 50 μg/l atrazine. To label cryosections, monoclonal antibodies from cell culture supernatants and ascites fluid with differing specificities for s-triazines were used in combination with the fluorescent dye Phycoerythrin and biotin-streptavidin amplification. The best results were observed with antibodies gained from mice immunized with an ametryn sulfoxide-BSA conjugate, regardless of the method employed for antibody production. The consequences with respect to herbicide metabolism and binding are discussed.
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