Pseudoexfoliation (PE) material has been studied pathologically. However, there are no reports about intraocular muscle function in pseudoexfoliation syndrome (PXS). We studied intraocular muscle function in 12 patients (70.3 ± 10.1 years of age) with ipsilateral PE material by measuring the time course of pupillary diameter change and accommodogram. Initial intraocular pressure (16.3 ± 4.2 mmHg) was significantly higher in eyes with PXS than in normal fellow eyes (13.1+3.5 mm Hg). The pupillary diameter of an affected eye was smaller than that of a fellow eye after bilateral topical application of tropicamide. Continuous near-point testing revealed no significant difference; accommodative contractile time, relaxation time, and/or accommodopattern showed different patterns from the fellow eye. The results suggest that intraocular muscle function is disturbed in PXS.