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Lipoxygenase Products Induce Neutrophil Activation and Increase Endothelial Permeability After Thrombin‐Induced Pulmonary Microembolism

 

作者: Marc Perlman,   Arnold Johnson,   William Jubiz,   Asrar Malik,  

 

期刊: Circulation Research  (OVID Available online 1989)
卷期: Volume 64, issue 1  

页码: 62-73

 

ISSN:0009-7330

 

年代: 1989

 

出版商: OVID

 

关键词: α-thrombin;lippxygenase products;HETE leukotriene B4;pulmonary microembolism;neutrophil activation;lung vascular injury;endothelial monolayer

 

数据来源: OVID

 

摘要:

We examined the mechanism of the neutrophil (PMN)-dependent increase in pulmonary vascular permeability to protein after thrombin-induced pulmonary microembolism. Humoral factors that activate PMNs after thrombin-induced pulmonary microemboUsm were characterized in pulmonary lymph obtained from unanesthetized sheep challenged with intravenous infusion of α-thrombin. Tune-dependent increases in PMN migration, aggregation, and superoxide anion (O2-) generation were induced by the puhnonary lymph obtained within 20 minutes after thrombin infusion. The puhnonary lymph neutrophil activating factors present in ether extracts of lymph had retention times of leukotriene B4(LTB4) and monohydroxyeicosatetraenoic acids (HETEs) by high-performance liquid chromatography. The postthrombin lymph samples containing the LTB4and HETEs increased PMN O2-generation and endothelial monolayer permeability to125I-albumin in the presence of PMNs layered on the endothelial monolayers. Control lymph samples replete with LTB4, 5-HETE, and 15-HETE induced increases in PMN O2-generation and endothelial monolayer permeability toI25I-albumin in the presence of PMNs layered on the endothelial monolayers. Maximal increases in PMN O2-production and endothelial permeability occurred when LTB4, 5-HETE, and 15-HETE were coincubated with PMNs, indicating a synergistic action of these mediators in inducing PMN activation. Endothelial monolayer permeability to125I-albumin did not increase with postthrombin lymph samples obtained after pretreatment with the 5-lipoxygenase inhibitor, L-651, 392. The results indicate that lipoxygenase products generated in the lungs after thrombin-induced microembolism contribute to increased endothelial permeability secondary to PMN activation.

 

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