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Comparative stainability of plant root cells with basic dye (methylene blue) in association with aluminum tolerance

 

作者: T. Wagatsuma,   T. Kawashima,   K. Tawaraya,  

 

期刊: Communications in Soil Science and Plant Analysis  （Taylor Available online 1988）
卷期: Volume 19, issue 7-12  

页码: 1207-1215

 

ISSN:0010-3624

 

年代: 1988

 

DOI:10.1080/00103628809368006

 

出版商: Taylor & Francis Group

 

关键词: stainability;methylene blue;plasmalemma;aluminum tolerance

 

数据来源: Taylor

 

摘要:

To investigate the relationship between aluminum tolerance and the binding capacity of the root plasmalemma for aluminum, root sections were stained with methylene blue and the blue color intensity of protoplasts was measured with a multi‐purpose graphic analyzer connected to a personal computer. Methylene blue stained both the cell wall and the protoplasts. Root sections killed in boiling water were stained with the same intensity as living root sections.

 

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