Immunofluorescent detection of Histoplasma capsulatum in primate experimental ocular histoplasmosis
作者:
MiyashiroJanet E.,
JesterJames V.,
DelmageJ. Michael,
SmithRonald E.,
期刊:
Current Eye Research
(Taylor Available online 1986)
卷期:
Volume 5,
issue 11
页码: 833-840
ISSN:0271-3683
年代: 1986
DOI:10.3109/02713688609029234
出版商: Taylor&Francis
数据来源: Taylor
摘要:
We have developed a polyclonal anti-Histoplasma capsulatum antibody to detect II. capsulatum antigens in ocular tissue. Antibodies were specific for II. capsulatum as determined by enzyme linked immunosorbant assay and immunoprecipitation. A total of 21 choroidal histoplasmic lesions in 4 primate eyes (Macaca speciosa and Macaca mulatta). taken at various times from 14 to 60 days after the internal carotid artery injection of yeast phase organisms, were evaluated. Using an indirect immunofluorescent technique, these antibodies stained yeast phase organisms within acute choroidal lesions at 14 days after infection. By 60 days intact organisms were no longer detected; occasional cells, however, contained intracellular inclusions that stained with these antibodies. Although yeast phase organisms are rapidly cleared from the primate choroid, these data indicate that residual H. capsulatum antigens may remain in choroidal lesions after the acute infectious stage. These findings are consistent with the hypothesis that H. capsulatum antigens play a role in the immunologic reactivation of atrophic choroidal scars in ocular histoplasmosis.
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