Serum deprivation‐induced apoptosis in cultured hippocampi is prevented by kainate
作者:
Santiago Rivera,
Sandrine Guillot,
Christopher Agassandian,
Yezekiel Ari,
Michel Khrestchatisky,
期刊:
NeuroReport
(OVID Available online 1998)
卷期:
Volume 9,
issue 17
页码: 3949-3953
ISSN:0959-4965
年代: 1998
出版商: OVID
关键词: Brain;Dentate gyrus;Picrotoxin;Programmed cell death;Protein synthesis inhibition
数据来源: OVID
摘要:
SERUM deprivation of hippocampal organotypic cultures induced cell death within 6 h in dentate gyrus granule cells and hilar interneurons whereas neurons from other hippocampal regions were spared. Dying neurons exhibited condensed chromatin in the nuclei, as revealed by cresyl violet, Hoescht staining, and electron microscopy. Cell death was abolished by cycloheximide. KA, an agonist of AMPA/KA receptors that induces depolarization, also prevented neuronal death. This effect was antagonized by the AMPA/KA receptor antagonist DNQX, but not by APV, an antagonist of NMDA receptors. PTX, a GABAAreceptor antagonist, reduced neuronal death by 50% after serum withdrawal. These data indicate that protein synthesis-dependent programmed cell death (PCD) occurs in the dentate gyrus upon trophic support withdrawal and suggest that neuronal activity contributes to cellular homeostasis.
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